Fig. 3: Levels of Bok protein in CRC patient cohort

a Representative western blotting comparing the levels of BOK in colorectal tumour (T) and matched normal (N) colonic tissue samples. β-Actin was used as a loading control. b Scatter plot depicting the protein levels of BOK in colorectal tumour and matched normal tissue. Differences between tumour and matched normal (n = 28) tissues were assessed by Wilcoxon signed-rank test. The mean, median and standard deviation (SD) were stated below the panels. *p ≤ 0.05 compared to normal tissue. c Scatter plot of the BOK protein levels following outlier identification analysis (for details, see Materials and methods section) in colorectal tumour and matched normal tissue. Differences between tumour and matched normal (n = 20) tissue were assessed by Wilcoxon signed-rank test. The mean, median and standard deviation (SD) were stated below the panels. *p ≤ 0.05 compared to normal tissue. d Scatter plot of the BOK protein levels in Stage II (n = 11) vs Stage III (n = 22) CRC patient tissue. Differences between levels of BOK protein in Stage II and Stage III CRC samples were assessed by Mann–Whitney U test. The mean, median and SD are stated below the panels