Fig. 4: Levels of ER stress proteins in a cohort of CRC patients

a Representative western blotting comparing the levels of ER stress proteins GRP78, GRP94 and Calreticulin in colorectal tumour (T) and matched normal (N) colonic tissue samples. β-Actin was used as a loading control. Scatter plots depicting the protein levels of GRP78 b, GRP94 d and Calreticulin e in colorectal tumour and matched normal (n = 27 for GRP78 and GRP94 and n = 24 for Calreticulin) tissues. Differences between tumour and matched normal tissues were assessed by Wilcoxon signed-rank test. The mean, median and standard deviation (SD) are stated below the panels. c Scatter plot of the GRP78 protein levels in Stage II (n = 11) vs Stage III (n = 20) CRC patient tissue. Differences between protein levels of GRP78 in Stage II and Stage III CRC samples were assessed by Mann–Whitney U test. The mean, median and SD are stated below the panels