Fig. 2: Impact of AF on cancer colony/sphere formation in vitro and tumor development in vivo | Cell Death & Disease

Fig. 2: Impact of AF on cancer colony/sphere formation in vitro and tumor development in vivo

From: Elimination of stem-like cancer cell side-population by auranofin through modulation of ROS and glycolysis

Fig. 2

a, b A549 and NCI-H460 cells were incubated with the indicated concentrations of AF. After 14 days, cell colonies were fixed, stained, and counted. Each bar represents mean ± SD, n = 3 separate experiments; *p < 0.05; **p < 0.01. c, d GSC11 and GSC23 cells were plated in six-well plates at a density of 800 cells per well, and treated with the indicated concentrations of AF. Tumor spheres were counted on day 12. Each bar represents mean ± SD, n = 3 separate experiments; **p < 0.01; #no sphere formed. e A549 cells were first treated with 4 μM AF for 24 h, and then the viable cells were inoculated subcutaneously into the flanks of the mice at 5 × 104 or 1 × 105 cells per injection site. The mice were observed for tumor development without further drug treatment

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