Fig. 6: Givinostat effect on HUVECs and perturbation studies.
From: Givinostat reduces adverse cardiac remodeling through regulating fibroblasts activation
a Representative images of capillary-like structures formed by HUVECs pre-treated with Noggin or BMP7 and exposed to Givinostat/FBs-conditioned media or PBS after hypoxia. Diagrams quantifying nodes, junctions, total length segments, branches, total length and total segment length formed by HUVECs using ImageJ angiogenesis software. Scale bars represent 200 μm. b Relative gene expression regarding EndMT mechanism (tgf-β, snail1, twist1/2, n-cad, e-cad, smad1/2/3, sox9) angiogenesis (hif-1α and vegf) and apoptosis (bcl2) in the different culture media. Error bars represent ± SEM. Student’s t test, *p < 0.05, **p < 0.01, #p < 0.001
