Table 2 Mouse and human primer sequences for quantitative RT-PCR analysis
From: Givinostat reduces adverse cardiac remodeling through regulating fibroblasts activation
Gene symbol | RefSeq Acc.number | Sense-forward primer | Antisense-reverse primer |
|---|---|---|---|
MOUSE | |||
anf | NM_008725.2 | TCTTCTTCGTCTTGGCCTTT | GACCTCATCTTCTACCGGCA |
bcl2 | NM_009741 | GAGTACCTGAACCGGCATCT | TTGTTTGGGGCAGGTTTGTC |
bmp2 | NM_007553 | GAAGTTCCTCCACGGCTTCT | AGATCTGTACCGCAGGCACT |
bmp4 | NM_007554 | AGCCAACACTGTGAGGAGTT | GGATGCTGCTGAGGTTGAAG |
bmp7 | NM_007557 | GTGGTATCGAGGGTGGAAGA | ACAAGGCCGTCTTCAGTACC |
brachiury | NM_009309 | GGTGCTGAAGGTAAATGTGTC | GGCTGTAATCTCTCCTCATTCTGG |
col1a1 | NM_007742.3 | CCTCAGGGTATTGCTGGACA | GAAGGACCTTGTTTGCCAGG |
col1a2 | NM_007743.2 | GGAACAAATGGGCTCACTGG | CAAGTCCTCTGGCACCTGTA |
col3a1 | NM_009930.2 | CCCAACCCAGAGATCCCATT | GGTCACCATTTCTCCCAGGA |
e-cadherin | NM_009864.2 | CTGGACCGAGAGAGTTACCC | GTGCTTGGGTTGAAGACAGG |
f4/80 | NM_010130.4 | CAACCTGCCACAACACTCTC | ATGAGCAGCTGTAGGATCCC |
gapdh | NM_001289726 | CACCATCTCCCAGGAGCGCAG | CCTTCTCCATGGTGGTGCAGAC |
gata4 | NM_001310610.1 | GGAAGACACCTCAATCTCGT | CACAGGCATTGTACAGGTAG |
il-1α | NM_010554.4 | ATGTATGCCTACTCGTCGGG | CAACTCCTTCAGCAACACGG |
il-1β | NM_008361.4 | TGACGGACCCCAAAAGATGA | TCTCCACAGCCACAATGAGT |
il-4 | NM_021283 | CGAGCTCACTCTCTGTGGTG | TGAACGAGGTCACAGGAGAA |
mef2 | NM_001170537.1 | TCAGTTGGGAGTTTGCACTA | TGGTGGTACGGTCTCTAGGA |
mmp9 | NM_013599.4 | AAAACCTCCAACCTCACGGA | GTGGTGTTCGAATGGTCTTT |
mhc-α | NM_001164171.1 | CCAACACCAACCTGTCCAAG | CTCGTCGTGCATCTTCTTGG |
mhc-β | NM_080728.2 | CCTGGAGAATGACAAGCAGC | GAGCTTCTTCTGCAGCTGAC |
n-cadherin | NM_007664.4 | TGGCTGAAAATAGACCCCGT | TTCCTGTCCCACTCATAGGC |
nkx2.5 | NM_008700 | CAGTGGAGCTGGACAAAGCT | TAGCGACGGTTCTGGAATCA |
p53 | NM_011640 | ACAGTCGGATATCAGCCTCG | GCTTCACTCGGGTCTTCAAA |
plgf | NM_008827 | GTTGGCTGTGCATTCCCAG | TACACCAGCTTCTCCATGGG |
sma | NM_007392.3 | CCTCTGGACGTACAACTGGT | GGTAGTCGGTGAGATCTCGG |
snail1 | NM_011427.2 | CGACTACCTAGGTCGCTCTG | CTGCTGGAAGGTGAACTCCA |
snail2 | NM_011415.2 | CGAACTGGACACACACACAG | AAAGGAGAGTGGAGTGGAGC |
tnf-α | NM_013693.3 | CGTCGTAGCAAACCACCAAG | GGCAGAGAGGAGGTTGACTT |
tnni3 | NM_009406.4 | AGCAGGTGAAGAAGGAGGAC | GCATCGATATTCTTGCGCCA |
twist1 | NM_011658.2 | GCCAGGTACATCGACTTCCT | CCAGACGGAGAAGGCGTAG |
twist2 | NM_007855.3 | AAGATCATCCCCACGCTCC | ATTGTCCATCTCGTCGCTCT |
tgf-β1 | NM_011577 | CAACCCAGGTCCTTCCTAAA | GGAGAGCCCTGGATACCAAC |
casp3 | NM_009810.3 | GAGCAGCTTTGTGTGTGTGTGA | TTCGGCTTTCCAGTCAGACT |
HUMAN | |||
bcl2 | NM_000633.2 | GCCCTGTGGATGACTGAGTA | GAAATCAAACAGAGGCCGCA |
e-cadherin | NM_004360.3 | ACAACAAGCCCGAATTCACC | GGTGTTCACATCATCGTCCG |
fibronectin | NM_212482.2 | CCCCATTCCAGGACACTTCT | AGGGTTCTTCATCAGTGCCA |
hif-1α | NM_001530.3 | ATTTTGGCAGCAACGACACA | GGGTGAGGGGAGCATTACAT |
sma | NM_001141945.1 | CTGCTGAGCGTGAGATTGTC | TCAAGGGAGGATGAGGATGC |
mmp9 | NM_004994.2 | CGCTACCACCTCGAACTTTG | ATAGGGTACATGAGCGCCTC |
n-cadherin | NM_001792.4 | AGGGATCAAAGCCTGGAACA | TTGGAGCCTGAGACACGATT |
smad1 | NM_005900.2 | GTACTTCCTCCTGTGCTGGT | TGGAAAAGTGGCGTTGAGTG |
smad2 | NM_005901.5 | GACACCAGTTTTGCCTCCAG | CTCTGTGGCTCAATTCCTGC |
smad3 | NM_001145104.1 | CTAGGGCTGCTCTCCAATGT | AAGACCTCCCCTCCGATGTA |
snail1 | NM_005985.3 | AAGCCTAACTACAGCGAGCT | GAGTCCCAGATGAGCATTGG |
snail2 | NM_003068.4 | AGCATTTCAACGCCTCCAAA | TGGTTGTGGTATGACAGGCA |
sox9 | NM_000346.3 | GGCAAGCTCTGGAGACTTCT | CGCGGCTGGTACTTGTAATC |
tgf-β1 | NM_000660.5 | CAGCAGGGATAACACACTGC | CATGAGAAGCAGGAAAGGCC |
twist1 | NM_000474.3 | AGTCTTACGAGGAGCTGCAG | ATCTTGCTCAGCTTGTCCGA |
twist2 | NM_057179.2 | AGAGCGACGAGATGGACAAT | CTAGTGGGAGGCGGACATG |
vegf | NM_001171624.1 | TCTACCTCCACCATGCCAAG | TGATGATTCTGCCCTCCTCC |
casp3 | NM_032991.2 | AAAATACCAGTGGAGGCCGA | GCACAAAGCGACTGGATGAA |
ChIP_sequences | |||
bmp7 | NM_007557.3 | GATGAGCCAGGTCCAAGAGT | AGCAACTAAGGGCTGTGCTA |
e-cadherin | NM_009864.3 | ACACGGAGGGAGAACAATGT | CCCCAAGTAGCAGCATCCTA |
nos3 | NM_008713.4 | TAGGAGAGGAGCAAGGGTGA | TACAGTGGGAGGGCTTCGAG |
