Fig. 4: 14–3–3ζ overexpression inhibited the viability and proliferation of T cells and deviated the differentiation of naive T cell from effector T cells (Teff) to regulatory T cells (Treg). | Cell Death & Disease

Fig. 4: 14–3–3ζ overexpression inhibited the viability and proliferation of T cells and deviated the differentiation of naive T cell from effector T cells (Teff) to regulatory T cells (Treg).

From: 14-3-3ζ delivered by hepatocellular carcinoma-derived exosomes impaired anti-tumor function of tumor-infiltrating T lymphocytes

Fig. 4

a Naive T cells were transfected with empty retro-virus (RV-NC) or overexpressing 14-3-3ζ retro-virus (RV-14-3-3ζ) then the mRNA level of 14-3-3ζ was detected by real-time PCR (n = 6). b, c Naive T cells were transfected with RV-NC or RV-14-3-3ζ then the protein level of 14-3-3ζ was detected by western blot (n = 6). Data were presented as the mean ± SEM. All experiments were performed in independent triplicate. d Flow cytometry assay of CD69 and Ki67 in CD3+ T cells transfected with empty retrovirus (RV-NC) (n = 6) or 14-3-3ζ-expressing RV (RV-14-3-3ζ) (n = 6). e Flow cytometric analyses of the proportions of polarizing T cells (CD4+IFN-γ+, CD4+Foxp3+, and CD8+IFN-γ+ T cells) induced from naive T cells transfected with RV-NC (n = 6) or RV-14-3-3ζ (n = 6) Data were presented as the mean ± SEM (*** P < 0.001)

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