Fig. 6: The upregulation of NOX5 expression was essential for the higher sensitivity of U937-NDRG2 cells to cisplatin.
From: NDRG2 contributes to cisplatin sensitivity through modulation of BAK-to-Mcl-1 ratio
a Human NOX5 expression was confirmed by quantitative RT-PCR. b U937-NDRG2 cells were transfected with scramble- or shNOX5-lentivirus. The knockdown efficiency of NOX5 expression was confirmed by immunoblotting, and apoptotic cell death was then analyzed by Annexin V/PI staining. c NOX5-knockdown cells were stained with DCFH-DA and ROS levels were determined by flow cytometry. d Active Bax (green) in the indicated experimental groups was visualized by confocal microscopy. U937-NDRG2 cells were treated with cisplatin for 12 h. e Levels of the indicated proteins were determined by immunoblotting. Ctrl: vehicle treatment or not infected group. ***p < 0.005 by t-test. Data are presented as mean ± SEM
