Fig. 4: MGL-deficient MEFs display features of oncogenic transformation.
From: Monoglyceride lipase gene knockout in mice leads to increased incidence of lung adenocarcinoma
a Left panel: MGL-deficient MEFs show increased cell proliferation and shortened cell doubling times. Equal numbers of MGL+/+, MGL+/− and MGL−/− MEFs (passage 15) (isolated from mice embryos of ~day 14 as described in Materials and methods) were cultured and counted at the indicated times. The presented data are based on experimental results collected from two independent experiments performed in triplicate. *P-value <0.1, **P-value <0.001. Right panel: MGL status of MEFs was confirmed by genomic DNA-PCR (upper panel) and western blot analyses (lower panel). b, c MGL-deficient (MGL+/− and MGL−/−) MEFs but not MGL-proficient (MGL+/+) MEFs form foci on cell culture plates (b) and exhibit anchorage-independent growth on soft agar (c)
