Fig. 3: Rps27l inactivation increases the levels of p53 and its targets in Trp53+/− mice. | Cell Death & Disease

Fig. 3: Rps27l inactivation increases the levels of p53 and its targets in Trp53+/− mice.

From: Inactivation of ribosomal protein S27-like confers radiosensitivity via the Mdm2-p53 and Mdm2–MRN–ATM axes

Fig. 3

a–c Inactivation of Rps27l induces the levels of p53 in small intestine (a, b) and spleen (c) of Trp53+/− mice upon radiation. Mice with indicated genotypes were irradiated at 8 Gy, and then small intestine (a, b) and spleen (c) were collected at indicated time points, followed by IHC staining with p53 antibody and photograph. Positive staining cells of each crypt were counted from at least three randomly selected microscopic fields (b). *p < 0.05. Scale bars represent 20 µm. d, e Inactivation of Rps27l induces p53 levels in multiple organs of Trp53+/− mice with or without radiation. The indicated organs from two individual male mice with indicated genotypes were homogenized and subjected to IB with indicated antibodies. f Inactivation of Rps27l induces the levels of p53 and its targets in Trp53+/− MEFs upon radiation. MEFs with indicated genotypes were irradiated at 5 Gy. Cells were then harvested at indicated time points post irradiation, followed by IB with indicated antibodies

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