Fig. 3: HSP60 associated with the phosphorylation of Erk1/2. | Cell Death & Disease

Fig. 3: HSP60 associated with the phosphorylation of Erk1/2.

From: Oncogenic HSP60 regulates mitochondrial oxidative phosphorylation to support Erk1/2 activation during pancreatic cancer cell growth

Fig. 3

a Analysis of mitochondrial-to-nucleus pathways in shHSP60 Panc-1 cells and control (Ctrl) cells. Cell lysates were analyzed by western blot with antibodies against P38, p-p38, Src, p-Src, Akt1, p-Akt (Thr308), p-Akt (Ser473), Erk1/2, p-Erk1/2, AMPKα, p- AMPKα, JNK, p-JNK, and actin. b–d Western blot analysis of p-Erk1/2 in the indicated cells and tissues: b shHSP60 Panc-1 cells vs. cells with rescued expression of HSP60, c cells from PDAC cell lines (SW1990, Patu-8988, and Panc-1) vs. adjacent normal pancreatic ductal cells (hTERT-HPNE), and d fresh PDAC tissue vs. paired normal tissue. N adjacent normal tissue; T tumor tissue

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