Fig. 2: Function of TRIM36 in PCa cell lines. | Cell Death & Disease

Fig. 2: Function of TRIM36 in PCa cell lines.

From: TRIM36, a novel androgen-responsive gene, enhances anti-androgen efficacy against prostate cancer by inhibiting MAPK/ERK signaling pathways

Fig. 2

a Expression of TRIM36 in prostate cancer cell lines. Top: Relative mRNA expressions of TRIM36 in PCa cell lines (C4–2, LNCAP, DU145, and PC-3) and the normal human prostate epithelial cell line RWPE-1. Bottom: Relative protein expression of TRIM36 in PCa cell lines (C4–2, LNCAP, DU145 and PC-3) and RWPE-1. b, c The knockdown and overexpression of TRIM36 were confirmed. b The knockdown and overexpression of TRIM36 in LNCAP and PC-3 cell lines were confirmed with RT-PCR to detect the relative mRNA expression of TRIM36 (*P < 0.05). c The knockdown and overexpression of TRIM36 in LNCAP and PC-3 cell lines were confirmed with western blot to detect the protein expression of TRIM36 (*P < 0.05). d–h TRIM36 regulates cell cycles and proliferation. d The knockdown of TRIM36 significantly promoted cell growth in the LNCAP line, while the overexpression of TRIM36 significantly inhibited cell growth in the PC-3 line. e–f The knockdown of TRIM36 prevented G0/G1 phase cell cycle arrest in the LNCAP line, while the overexpression of TRIM36 resulted in G0/G1 phase cell cycle arrest in the PC-3 line. g, h The knockdown of TRIM36 obviously increased colony formation efficiency in the LNCAP line, while the overexpression of TRIM36 decreased colony formation efficiency in the PC-3 line

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