Fig. 4: High LanCL1 expression promotes proliferation of prostate cancer cells in vitro and in vivo. | Cell Death & Disease

Fig. 4: High LanCL1 expression promotes proliferation of prostate cancer cells in vitro and in vivo.

From: LanCL1 protects prostate cancer cells from oxidative stress via suppression of JNK pathway

Fig. 4: High LanCL1 expression promotes proliferation of prostate cancer cells in vitro and in vivo.The alternative text for this image may have been generated using AI.

a Stably transfected LNCaP cells and PC-3 cells interfered with LanCL1 were established. LanCL1 was knockdown by siRNA as indicated. The efficiency of knockdown were examined by western blotting. Tubulin served as the control. b Cell proliferation was measured at the indicated time points by MTS colorimetric assay (absorbance at 490 nm (OD490). Student’s t-test was performed for statistical significance analysis. N>3. *P<0.05, **P<0.01, ***P<0.001 c Tumor growth in LanCL1 overexpressed PC-3 cells and control cells was investigated by xenograft tumor models. Student’s t-test was performed for statistical significance analysis. N = 7. **P<0.01, ***P<0.001. d The cell cycle distribution was analyzed by fluorescent-activated cell scanning (FACS) in LanCL1 overexpressed LNCaP cells. e Column chart indicates the mean and SEM of the S-phase fraction for each cell. Student’s t-test was performed for statistical significance analysis. N = 5

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