Fig. 3: EZH2 is reactivated in emergent clones.
From: Regulation of senescence escape by the cdk4–EZH2–AP2M1 pathway in response to chemotherapy
a LS174T cells have been stimulated with the indicated concentrations of Palbociclib for 48 h. EZH2 expression was analyzed by western blot (n = 3). b LS174T cells have been transfected with the indicated siRNAs for 48 h. EZH2 expression was analyzed by western blot (n = 3). c LS174T cells have been stimulated with Palbociclib for 48 h. ChIP assays have been performed using the indicated primers and analyzed by quantitative PCR (n = 3). d Cells have been treated with sn38 (5 ng/ml) for 4 days. EZH2 expression was evaluated by western blot analysis (n = 4). e Following cell sorting, EZH2 expression has been evaluated by quantitative RT-PCR and by western blot in the dividing PLD and senescent PLS subpopulations (n = 6; **p < 0.01; n=4, *p < 0.05). f Analysis of EZH2 and Ki67 expression by immunofluorescence in PLC emergent cells (n = 4)
