Fig. 5: CBLB and GRB2 are direct targets of miR-27b.

a Predicted sequences binding regions between wild-type (wt) or mutant (mut) 3′-UTRs of CBLB/GRB2 and miR-27b. b The luciferase activities were measured in Bads-200 cells co-transfected with miR-27b and specific luciferase vectors. c, d, e The mRNA and protein levels of CBLB/GRB2 in BCap37 and Bads-200 cells were analyzed. f Representative images of tumor samples in Bads-200-xenograft that were stained with CBLB and GRB2 by IHC. Scale bars: (main) 100 μm; (insets) 25 μm