Fig. 3: EZH2 positively regulates VSMC growth. | Cell Death & Disease

Fig. 3: EZH2 positively regulates VSMC growth.

From: EZH2 inhibits autophagic cell death of aortic vascular smooth muscle cells to affect aortic dissection

Fig. 3

a Protein level of EZH2, H3K27me2, and H3K27me3 were detected by western blot in VSMCs infected with lenti-shRNA or lenti-shEZH2. Left panel, representative western blots; right panel, quantitative results. GAPDH serves as a loading control (n = 4). b Growth curve of VSMC-infected lenti-shRNA or lenti-shEZH2 (n = 3). c Representative images of VSMCs under light microscopy. *p < 0.05 vs. lenti-shRNA in a and b. d The western blots of Flag, EZH2, H3K27me2, and H3K27me3 in the samples of VSMCs overexpressed GFP or EZH2 (left panel), and quantitative results of these blots showed at the right panel (n = 4). e The VSMC number was counted at indicated time point after lenti-GFP or lenti-EZH2 infected (n = 3). f Representative images of VSMCs under light microscopy. *p < 0.05 vs. lenti-GFP in d and e

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