Fig. 3: CFTR alters the levels of liver injury biomarker and autophagy in CBS+/− mice. | Cell Death & Disease

Fig. 3: CFTR alters the levels of liver injury biomarker and autophagy in CBS+/− mice.

From: Homocysteine activates autophagy by inhibition of CFTR expression via interaction between DNA methylation and H3K27me3 in mouse liver

Fig. 3

CBS+/− mice injected with lentivirus Lv-CFTR or Lv-GFP through the tail vein (see Materials and Methods). a CFTR mRNA and protein levels were determined by qRT-PCR and western blot respectively. Data are presented as mean ± s.d. relative to Lv-GFP-injected mice. b Circulating levels of ALT and AST were analyzed. Results are means ± s.d. (n = 6). *P < 0.05. c Liver section from mice were subjected to H&E (upper) and Oil Red O (middle) staining. Transmission electron microscope (TEM) (under) was used to analyse cell autophagy in liver of CBS+/− mice. d and e mRNA and protein expression of p62, BECN1, LC3 and Atg12 were determined by qRT-PCR and western blot respectively in the liver of CBS+/− mice after injection with lentivirus expressing CFTR

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