Table 1 Primer sequences for qRT-PCR
Gene | Species | Primer Sequence (5′ → 3′) | Length (bp) | Annealing Temperature (°C) |
|---|---|---|---|---|
CFTR | Human | F: AGAACTGAAACTGACTCGGAAGGR: GCAGAATGAGATGGTGGTGAA | 157 | 59.0 |
| Â | Mouse | F: ACAGGATAGAAGCGATGTTGGATTGCR: GAGGCTTGTGCTTGCTGGAGTG | 177 | 58.5 |
DNMT1 | Human | F: GCGGCAGACCATCAGGCATTCR: CGTTCTCCTTGTCTTCTCTGTCATCC | 140 | 60.0 |
| Â | Mouse | F: TCACTTGGACGAGGACGAGGACR: TACCTGCTCTGGCTCTGCTTCC | 115 | 57.5 |
DNMT3a | Human | F: GGAGCCACCAGAAGAAGAGAAGAATCR: TGCCGCACCTCGTACACCAG | 189 | 57.5 |
| Â | Mouse | F: AACTGCTTCTTGGAGTGTGCTTACCR: GGTCTTCCTTAATGGCTGCCTGAG | 184 | 59.0 |
DNMT3b | Human | F: AGCTCTTACCTTACCATCGACCTCACR: TACTCTGAACTGTCTCCATCTCCACTG | 164 | 57.5 |
| Â | Mouse | F: GAAGGTGCGTCGTTCAGACAGTAGR: TCAGAAGCAGCAGAGTCATTGGTTG | 93 | 58.0 |
EZH2 | Human | F: CATTCGGTAAATCCAAACTGCR: CGACATACTTCAGGGCATCA | 148 | 59.0 |
| Â | Mouse | F: AGAGTGGAAGCAGCGGAGGATACR: CATTATAGGCACCGAGGCGACTG | 157 | 56.5 |
p62 | Human | F: GCTGTGGATGAAGTGGAACCTCTACR: CTGGCTGGAAGTCAGGCTGTAAC | 109 | 56.8 |
| Â | Mouse | F: ACATACGCAGAACAGAGTTACGAAGGR: CATTCCAGTCATCTTGTCCGTAGGC | 133 | 58.0 |
BECN1 | Human | F: ATGGAAGGGTCTAAGACGTCCAACAR: CGCCTGGGCTGTGGTAAGT | 156 | 55.7 |
| Â | Mouse | F: GACGAACTCAAGAGTGTGGAGAACCR: AGATGTGGAAGGTGGCATTGAAGAC | 97 | 58 |
LC3B | Human | F: ATGAAGATGAGATTCTTCAGTTCTCR: TCGTCTTTCTCCTGCTCGTAG | 326 | 57.4 |
| Â | Mouse | F: GCTGGTGCTGCCTATGTTGTCTCR: AATACATGGTCCTGTGGCAAGATTCC | 146 | 56.5 |
Atg12 | Human | F: ATGACTAGCCGGGAACACCAAGTTTR: TCCTCCGCCATCTTGCTTG | 152 | 56.5 |
| Â | Mouse | F: TGCTGAAGGCTGTAGGAGACACTCR: TGATGAAGTCAATGAGTCCTTGGATGG | 93 | 57.0 |
GAPDH | Human | F: CGGATTTGGTCGTATTGGGR: CGCTCCTGGAAGATGGTGAT | 213 | 56 |
| Â | Mouse | F: CCCTTAAGAGGGATGCTGCCR: ACTGTGCCGTTGAATTTGCC | 263 | 55.0 |
