Fig. 4: Tumor tissue sections of NIC-treated mice showed enhanced expression of both EZH2 and Ki67. | Cell Death & Disease

Fig. 4: Tumor tissue sections of NIC-treated mice showed enhanced expression of both EZH2 and Ki67.

From: Nicotine associated breast cancer in smokers is mediated through high level of EZH2 expression which can be reversed by methyltransferase inhibitor DZNepA

Fig. 4

a Immunohistochemistry in tissue sections prepared from tumors collected from vehicle, NIC, and NIC and DZNepA xenografts shows the effect of drugs on the expression of EZH2 and Ki67. Insets show 10 times digitally magnified pictures of images captured with ×40 objective. b, c Graph depicts the percentage of EZH2- and Ki67-positive cancer cells in the three groups. d Western blot shows the EZH2 expression level in tumor of three groups. One-way ANOVA was used for statistical analysis *P < 0.05, **P < 0.005, ***P < 0.001. Scale bar 50 µm

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