Fig. 3: Knockdown of OPN reduces ICC growth and metastasis in vivo. | Cell Death & Disease

Fig. 3: Knockdown of OPN reduces ICC growth and metastasis in vivo.

From: Osteopontin promotes metastasis of intrahepatic cholangiocarcinoma through recruiting MAPK1 and mediating Ser675 phosphorylation of β-Catenin

Fig. 3

a ICC cell lines with OPN knockdown and control were injected subcutaneously into nude mice (one mouse died 3 weeks later both in HuCCT1-shNC group and HuCCT1-shOPN group). The dynamic change of tumor volume in subcutaneous models was shown at 6 weeks after injection. b Knockdown of OPN decreased ICC metastasis in vivo. Stable OPN knockdown cell lines were injected into the intraperitoneal cavity of nude mice (n = 6), mice were sacrificed after 6 weeks, and the number of mesenteric lymph nodes metastasis per mouse was counted. (c, d) Down-regulation of OPN significantly inhibited lung (c) and liver (d) metastasis in ICC nude mice models. Scale bar = 200 μm

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