Fig. 3: Effect of L-BMAA-induced PC on motor neurons survival.

Representative image of Nissl stained in spinal cord (a). Scale bar 200 μm. Cell counting analysis of motor neurons expressed as the percentage of total motor neurons in spinal cord of 4.5 month-old SOD1 G93A mice treated with vehicle or l-BMAA compared to wild-type vehicle (b). Representative image of Nissl stained in nucleus facialis (c). Scale bar 200 μm. Cell counting analysis of motor neurons expressed as the percentage of total motor neurons in nucleus facialis of 4.5 month-old SOD1 G93A mice treated with vehicle or l-BMAA compared to wild-type vehicle (d). Data are expressed as mean ± SEM (n = 4 for each group). *P < 0.05 vs. wild-type vehicle **P < 0.05 vs. all other experimental groups. P values were obtained using one-way ANOVA with Newman Keuls’s correction for multiple comparisons