Fig. 4: Hemopexin knockout mice showed reduced functional recovery of hindlimb after SCI. | Cell Death & Disease

Fig. 4: Hemopexin knockout mice showed reduced functional recovery of hindlimb after SCI.

From: Plasma Hemopexin ameliorates murine spinal cord injury by switching microglia from the M1 state to the M2 state

Fig. 4

Functional recovery was assessed by determining the Basso Mouse Scale (BMS) scores (a), Basso Mouse Scale subscores (b) and swimming tests (c, d) over a 35-day period (n = 15 mice per group). e Schematic graph of the thoracic spinal cord (T8–T9) crush model used. f Representative images of GFAP immunostaining in spinal cord sections at the epicenter and peri-lesion sites from Hpx−/− and Hpx+/+ mice on 35 dpl after SCI. The white line denotes the size of the lesion. Quantification of GFAP immunostaining in the total tissue area (g), GFAP-negative area (h), lesion area (i), and spared tissue (j). The relationship between spared white matter and final BMS score of each mouse was assessed with Pearson’s regression analysis (k). l Representative images of 5-HT+ fibers (red) co-stained with GFAP (blue) in sagittal sections. m Quantification reveals a significant decrease in 5-HT+ fiber sprouting caudal to the injury in Hpx−/− SCI group vs. Hpx+/+ SCI group mice on 35 dpl. a–f 5-HT+ fibers in the boxed areas are enlarged in the right panels in two groups. Dashed lines indicate lesion margins. (n = 5 mice per group). *p < 0.05, **p < 0.01, ***p < 0.005, compared with the indicated control. Scale bar = 100 µm

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