Fig. 3: GAS5 inhibited transcriptional of EZH2, and knockdown of GAS5 suppressed GA-induced inhibition of EZH2 transcription. | Cell Death & Disease

Fig. 3: GAS5 inhibited transcriptional of EZH2, and knockdown of GAS5 suppressed GA-induced inhibition of EZH2 transcription.

From: Long noncoding RNA GAS5 promotes bladder cancer cells apoptosis through inhibiting EZH2 transcription

Fig. 3

a GV144-GAS5 or the empty vector was stably transfected into BC cells, EZH2 protein levels were detected by Western-blot assay. b GAS5 shRNA or the control (nontarget shRNA) was stably transfected into BC cells, and 2.0 μM GA or isopyknic PBS was treated for 48 h, the Western-blot assay indicated that GAS5 shRNA evidently inhibited GA-induced downregulation of EZH2 protein expression and the activation of caspase-3. c BC cells stably transfected with GV144-GAS5 or the empty vector were collected, and EZH2 mRNA levels were assessed by RT-qPCR assay. d BC cells stably transfected with GAS5 shRNA or the control shRNA (nontarget shRNA) were treated with 2.0 μM GA or isopyknic PBS for 48 h, RT-qPCR showed that GAS5 shRNA remarkably reversed the inhibition of EZH2 mRNA expression by GA. e EZH2 transcriptional activities of BC cells stably transfected with GV144-GAS5 or the empty vector were assessed by luciferase reporter assay. f The cells were then treated with 2.0 μM GA or isopyknic PBS. Forty hours later, EZH2 transcriptional activities were analyzed, the activity of firefly luciferase was normalized by renilla luciferase. Results were presented as the means ± SD of triplicates. *P < 0.05 versus controls, #a significant difference between GAS5 shRNA and nontarget shRNA groups

Back to article page