Fig. 5: The rs7119 SNP within the HMG20A 3′ UTR decreases protein levels.
From: The type 2 diabetes-associated HMG20A gene is mandatory for islet beta cell functional maturity
Expression profile of putative miRNAs binding to the “mut” allele of the HMG20A transcript in a human 1.1E7 cells and b mouse islets. Data are presented respect to miR375 expression levels. c Schematic representation of the HMG20A gene depicting the promoter region (HMG20A promoter), transcription starting site (tss) and 11 exons. Gaussia luciferase (GLuc) reporter constructs harboring the 3′ UTR corresponding to either wild type (wt) or rs7119 mutant (mut) allele under pSV40 promoter are shown below. d HMG20A mRNA levels assessed by qPCR in the human pancreatic beta cell line 1.1E7 cultured in 24 mM glucose for 24, 48, 72, or 96 h (n = 5 independent experiments performed in triplicates). e Human pancreatic beta 1.1E7 and f RPE1 cell lines were transfected with the reporter construct bearing either wt or rs7119 mut variant of the HMG20A 3′ UTR. Both the Gaussia Luciferase activity and SEAP activity were determined 24 h post transfection (n = 3 independent experiments). Data are represented as means + S.E.M. p values were determined by ordinary one-way ANOVA (d) or unpaired Student's t-test (e, f). *p < 0.05
