Fig. 1: Canagliflozin is cytotoxic for RPTEC/TERT1 cells and causes aerobic glycolysis at subtoxic concentrations. | Cell Death & Disease

Fig. 1: Canagliflozin is cytotoxic for RPTEC/TERT1 cells and causes aerobic glycolysis at subtoxic concentrations.

From: Canagliflozin mediated dual inhibition of mitochondrial glutamate dehydrogenase and complex I: an off-target adverse effect

Fig. 1

a, b, e Differentiated (day 16) or c, d proliferating RPTEC/TERT1 cells were exposed to the different gliflozins in the presence of 0.5% DMSO for 24 h followed by quantification of a, c cell viability via MTT reduction or b, d cytotoxicity via LDH leakage. MTT reduction is normalized to non-treated cells. e Ratio of secreted lactate divided by consumed glucose in differentiated RPTEC/TERT1 cells as indicator of aerobic glycolysis following 24 h of exposure to gliflozins at subtoxic concentrations. Data are mean ± SEM of at least three independent experiments performed in duplicate. *P < 0.05 relative to 0.5% DMSO (solvent control; s.c.) assessed by one-way analysis of variance (ANOVA) with Dunnett’s post-test

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