Table 2 Effect of 24 h of incubation of alveolar basal epithelial A549 and microglial BV-2 cells with engineered carbon nanodiamonds (ECNs) on purine and pyrimidine nucleotides (mono, di, and triphosphorylated)

From: Non-toxic engineered carbon nanodiamond concentrations induce oxidative/nitrosative stress, imbalance of energy metabolism, and mitochondrial dysfunction in microglial and alveolar basal epithelial cells

 

GTP (nmol/mg protein)

GDP (nmol/mg protein)

GMP (nmol/mg protein)

IMP (nmol/mg protein)

UTP (nmol/mg protein)

UDP (nmol/mg protein)

UMP (nmol/mg protein)

CTP (nmol/mg protein)

CDP (nmol/mg protein)

CMP (nmol/mg protein)

Resting A549

25.71 (8.44)

4.51 (1.13)

0.64 (0.10)

0.40 (0.05)

39.56 (4.05)

0.58 (0.09)

0.18 (0.04)

26.32 (3.47)

0.52 (0.12)

0.047 (0.018)

A549 + ECNs

16.14* (2.07)

5.69* (1.38)

0.96* (0.12)

0.49* (0.07)

32.73* (3.71)

1.20* (0.38)

0.16* (0.03)

20.10* (2.21)

0.76* (0.17)

0.043* (0.011)

Resting BV-2

21.79 (5.13)

6.73 (2.02)

2.05 (0.79)

2.56 (0.31)

45.83 (6.25)

3.09 (0.89)

0.77 (0.26)

16.34 (3.32)

2.45 (0.68)

1.37 (0.45)

BV-2 + ECNs

17.32* (3.37)

6.01* (1.51)

4.85* (0.74)

2.28* (0.56)

33.36* (8.13)

4.58* (1.64)

0.59* (0.28)

10.92* (2.45)

1.95* (0.72)

1.32* (0.19)

  1. Values are the mean of four different experiments. Standard deviations are in parenthesis. Incubation conditions and HPLC method to separate simultaneously the indicated compounds are given in Materials and methods
  2. ECNs engineered carbon nanodiamonds, GTP, GDP, GMP guanosine mono, di, triphosphate, respectively; IMP inosine monophosphate, UTP, UDP, UMP uridine mono, di, triphosphate, respectively; CTP, CDP, CMP cytidine mono, di, triphosphate, respectively
  3. *Significantly different from resting, p < 0.01
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