Fig. 10: Decreased PED6 β, increased cGMP, and increased TUNEL staining in rod-cre PKM2 KO mouse retinas.
From: Pyruvate kinase M2 regulates photoreceptor structure, function, and viability
Prefer-fixed sections of wild-type a and rod-cre PKM2-KO c mouse retinas were subjected to immunofluorescence with anti-PDE6β a, c antibody. b, d Omission of primary antibody. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; ONL, outer nuclear layer; OPL, outer plexiform layer; RIS, rod inner segments; ROS, rod outer segments. Scale bar = 50 μm. Cryosections of wild-type e, f and rod-cre PKM2-KO g, h mouse retinas were subjected to immunofluorescence with anti-cGMP e–h antibody. f, h cGMP (green)/DAPI (blue) staining. i Omission of primary antibody. Prefer-fixed retinal sections of PKM2-WT and rod-cre PKM2-KO mice were examined for cell death with in situ localization of apoptosis using TUNEL j, k. TUNEL-positive cells were counted on the entire retina l. Data are mean ± SEM, (n = 3). *p < 0.001
