Fig. 13: Determination of NADPH and NADP levels in wild-type and rod-cre PKM2-KO mouse retinas.
From: Pyruvate kinase M2 regulates photoreceptor structure, function, and viability
Mouse retinal tissues from wild-type and rod-cre PKM2-KO mice were used to measure NADPH and NADP levels a and their ratio was presented b. Data are mean ± SEM (n = 3). *p < 0.05. PKM2-WT and rod-cre PKM2-KO mouse retinas were placed in Ringer solution and incubated in the presence of radiolabeled [2-3H] glycerol for 45 min at room temperature. Total lipids were extricated and subjected to TLC to separate phospholipids c and triglycerides d followed by counting the radioactivity. Data mean ± SEM (n = 4)
