Fig. 3: NOR-promoted Treg differentiation is independent of miRs under hypoxic microenvironment. | Cell Death & Disease

Fig. 3: NOR-promoted Treg differentiation is independent of miRs under hypoxic microenvironment.

From: Norisoboldine, a natural AhR agonist, promotes Treg differentiation and attenuates colitis via targeting glycolysis and subsequent NAD+/SIRT1/SUV39H1/H3K9me3 signaling pathway

Fig. 3

a CD4+ T cells were cultured with anti-CD3/CD28 (2 µg/ml), NOR (1, 3, 10, 30 μM), and TCDD (5 nM) in hypoxia for 24 h. The mRNA expressions of miR-31, miR-219, and miR-490 were analyzed by Q-PCR. b CD4+ T cells were transfected with miR-31 or NC mimic and mRNA expression of miR-31 was analyzed by Q-PCR. c CD4+ T cells were transfected with miR-31 or NC mimic, followed with incubation of anti-CD3/CD28 (2 µg/ml), NOR (30 μM), and TCDD (5 nM) in hypoxia for 72 h, and frequencies of Treg cells were analyzed by flow cytometry. Data were expressed as means ± SEM of three independent experiments. **P < 0.01 vs. Normal group

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