Fig. 5: NOR decreases NAD+ and SIRT1 levels in CD4+ T cells under hypoxic microenvironment. | Cell Death & Disease

Fig. 5: NOR decreases NAD+ and SIRT1 levels in CD4+ T cells under hypoxic microenvironment.

From: Norisoboldine, a natural AhR agonist, promotes Treg differentiation and attenuates colitis via targeting glycolysis and subsequent NAD+/SIRT1/SUV39H1/H3K9me3 signaling pathway

Fig. 5

a CD4+ T cells were cultured with anti-CD3/CD28 (2 µg/ml), NOR (1, 3, 10, 30 μM), and TCDD (5 nM) in hypoxia for 48 h, and NAD+ level was analyzed by using kits. b, c CD4+ T cells were pretreated with CH223191 (10 μM) for 2 h or transfected with HK2 plasmid/siAhR-3, followed with incubation of anti-CD3/CD28 (2 µg/ml), NOR (1, 3, 10, 30 μM), and TCDD (5 nM) in hypoxia for 48 h. NAD+ level was analyzed by using kits. d, e CD4+ T cells were cultured with anti-CD3/CD28 (2 µg/ml), NOR (1, 3, 10, 30 μM), and TCDD (5 nM) in hypoxia for 48 h. The mRNA levels of SIRT1 and 2 were analyzed by Q-PCR d; the protein level of SIRT1 was analyzed by western blotting e. f, g CD4+ T cells were pretreated with CH223191 (10 μM) for 2 h or transfected with HK2 plasmid/siAhR-3, followed with incubation of anti-CD3/CD28 (2 µg/ml), NOR (30 μM) and TCDD (5 nM) in hypoxia for 48 h. The mRNA and protein levels of SIRT1 were analyzed by Q-PCR and western blotting, respectively. Data were expressed as means ± SEM of three independent experiments. *P < 0.05, **P < 0.01 vs. Normal group; $P < 0.05, $$P < 0.01 vs. NOR (30 μM) group

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