Fig. 6: NOR facilitates the ubiquitin-proteasomal degradation of SUV39H1 protein in CD4+ T cells under hypoxic microenvironment. | Cell Death & Disease

Fig. 6: NOR facilitates the ubiquitin-proteasomal degradation of SUV39H1 protein in CD4+ T cells under hypoxic microenvironment.

From: Norisoboldine, a natural AhR agonist, promotes Treg differentiation and attenuates colitis via targeting glycolysis and subsequent NAD+/SIRT1/SUV39H1/H3K9me3 signaling pathway

Fig. 6

a–c CD4+ T cells were cultured with anti-CD3/CD28 (2 µg/ml), NOR (1, 3, 10, 30 μM), and TCDD (5 nM) in hypoxia for 48 h. The protein levels of MLL1, G9a, SUV39H1, JMJD3, and EZH2 were analyzed by western blotting a; the mRNA level of SUV39H1 was analyzed by Q-PCR b; the turnover of SUV39H1 protein was analyzed by pulse-chase experiment c. d CD4+ T cells were cultured with anti-CD3/CD28 (2 µg/ml), MG132 (5 μM), NOR (30 μM), and TCDD (5 nM) in hypoxia for 48 h, and the protein level of SUV39H1 was analyzed by western blotting. e CD4+ T cells were cultured with anti-CD3/CD28 (2 µg/ml), NOR (1, 3, 10, 30 μM), and TCDD (5 nM) in hypoxia for 48 h, and the ubiquitination level of SUV39H1 was analyzed. f–h CD4+ T cells were pretreated with EX-527 (1 μM)/CH223191 (10 μM) for 2 h or transfected with HK2 plasmid/siAhR-3, followed with incubation of anti-CD3/CD28 (2 µg/ml), NOR (30 μM), and (5 nM) in hypoxia for 48 h. The protein level of SUV39H1 was analyzed by western blotting. Data were expressed as means ± SEM of three independent experiments

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