Fig. 1: GRK5 is highly expressed in NSCLC cancerous tissues.

a, b′ NSCLC tissue microarrays (TMAs) contained sections of 539 NSCLC cancerous tissues and 99 normal non-cancerous lung tissues were stained by GRK5 antibodies. Representative images with differential magnification (a and b×200; a′ and b′×200) of IHC staining were shown. a-a′ Representative images of both negative and positive GRK5 expression in normal lung tissues were shown. b-b′ Representative images of both negative and positive GRK5 expression in NSCLC tumor tissues were shown. c Quantification data for IHC staining result, the GRK5-positive staining percentages in both normal and NSCLC tissues were shown. p < 0.0001. Normal: normal lung tissues; Tumor: NSCLC cancerous tissues. d The percentage for GRK5-positive staining was higher in female and ADC NSCLC cancerous tissues, respectively. e, f Kaplan−Meier plotter was used to analyze the protein expression data from IHC (e) and transcriptional RNA data (f). GRK5 high expression in NSCLC had a significantly worse survival rate than low expression patients. Different color lines correlated with different GRK5 protein (e; p = 0.002) or RNA (f; p = 0.001) expression levels