Fig. 10: MEG3 inhibits the exptrssion of C-myc and CyclinD1 through β-catenin. | Cell Death & Disease

Fig. 10: MEG3 inhibits the exptrssion of C-myc and CyclinD1 through β-catenin.

From: Long noncoding RNA MEG3 suppresses liver cancer cells growth through inhibiting β-catenin by activating PKM2 and inactivating PTEN

Fig. 10

A LEF/TCF4 luciferase activity assay in liver cancer cells Hep3B cell lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-MEG3, respectively. Data are means of values from three independent experiments, mean ± SEM. **, P < 0.01; *, P < 0.05. B Chromatin immunoprecipitation (CHIP) with anti-TCF4 followed by PCR with C-Myc promoter and CyclinD1 promoter primers in liver cancer cells Hep3B cell lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-MEG3, respectively. IgG CHIP as negative control. C-Myc promoter and CyclinD1 promoter as INPUT. C C-myc promoter luciferase activity assay in liver cancer cells Hep3B. D CyclinD1 promoter luciferase activity assay in liver cancer cells Hep3B. E RT-PCR and Western blotting analysis of C-myc and CyclinD1 in liver cancer cells Hep3B cell lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-MEG3, pCMV6-A-GFP-MEG3 plus pCMV6-XL5-β-catenin, respectively. β-actin as an internal control

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