Fig. 7: MEG3 inhibits the interaction between GSK3β(Try216) and β-catenin. | Cell Death & Disease

Fig. 7: MEG3 inhibits the interaction between GSK3β(Try216) and β-catenin.

From: Long noncoding RNA MEG3 suppresses liver cancer cells growth through inhibiting β-catenin by activating PKM2 and inactivating PTEN

Fig. 7

A Western blotting analysis with anti-METTL3 in liver cancer cells Hep3B cell lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-MEG3, respectively. β-actin as an internal control. B RNA immunoprecipitation (RIP) with anti-METTL3 followed by RT-PCR with PTEN primers. IgG RIP as negative control. RT-PCR for PTEN as INPUT. C RNA immunoprecipitation (RIP) with anti-m6A followed by RT-PCR with PTEN primers. IgG RIP as negative control. RT-PCR for PTEN as INPUT. D PTEN 3′-UTR luciferase activity assay. Data are means of values from three independent experiments, mean ± SEM. **, P < 0.01; *, P < 0.05. E Western blotting analysis with anti-PTEN and RT-PCR with PTEN primers. F Western blotting analysis with anti-AKT, anti-pAKT, anti-GSK3β(ser9), anti-GSK3β(Tyr216), anti-GSK3β, and anti-β-catenin in liver cancer cells Hep3B cell lines. G Co-immunoprecipitation (IP) with anti-GSK3β(Tyr216) followed by Western blotting with anti-β-cateninin. IgG IP as negative control. INPUT refers to Western blotting with anti-β-catenin

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