Fig. 3: The role of AMPK in FGF21-induced protection in cardiomyocytes against HG/ Pal. | Cell Death & Disease

Fig. 3: The role of AMPK in FGF21-induced protection in cardiomyocytes against HG/ Pal.

From: Fibroblast growth factor-21 prevents diabetic cardiomyopathy via AMPK-mediated antioxidation and lipid-lowering effects in the heart

Fig. 3

Primary cardiomyocytes were isolated from neonatal mice and treated with either control or AMPK-specific siRNA and then the cells were cotreated with both HG/Pal and FGF21 for 24 h. Western blotting was used to detect the phosphorylation (a, b) and expression levels of AMPK (a, c). Under this circumstance, the expressions of cleaved-caspase 3 (d), ANP (e), CTGF (f), and 4-HNE (g) were measured by western blot assay. The translocation between the nuclei and cytosol of Nrf2 (h, i) was evaluated by measuring the protein of each in the nuclei and cytosol, respectively. Data were collected from at least three independent experiments and presented as mean ± SD. *P < 0.05 vs. control in the Con-siRNA group; #P < 0.05 vs. HG/Pal in the Con-siRNA group; @P < 0.05 vs. control in AMPK-siRNA group; $P < 0.05 vs. HG/Pal/FGF21 in the Con-siRNA group

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