Fig. 1: The interaction between CAPN7 and HOXA10. | Cell Death & Disease

Fig. 1: The interaction between CAPN7 and HOXA10.

From: Calpain7 impairs embryo implantation by downregulating β3-integrin expression via degradation of HOXA10

Fig. 1: The interaction between CAPN7 and HOXA10.The alternative text for this image may have been generated using AI.

a Immunoblot analysis of whole cell lysates and anti-Myc immunoprecipitation of HEK293T cells transfected with Flag-CAPN7 alone or together with Myc-HOXA10. b Immunoblot analysis of whole-cell lysates and anti-Flag immunoprecipitation of HEK293T cells transfected with Myc-HOXA10 alone or together with Flag-CAPN7. c, d Immunoblot analysis of Ishikawa whole-cell lysates and anti-HOXA10 immunoprecipitation. Goat IgG was used as a negative control for the co-IP procedure. e Schematic representation of the HOXA10 mutants used in f. f Immunoblot analysis of whole-cell lysates and anti-Flag immunoprecipitations of HEK293T cells transfected with Flag-CAPN7 and various Myc-HOXA10 constructs. g Cellular localization of Flag-HOXA10 and GFP-CAPN7. Flag-HOXA10 and GFP-CAPN7 localized primarily to the nucleus and the colocalization of the two proteins was apparent in the merged image. h Cellular localization of endogenous HOXA10 and CAPN7 in the nuclear of Ishikawa cells

Back to article page