Fig. 3: CAPN7 downregulates the expression of HOXA10 and inhibits embryo implantation. | Cell Death & Disease

Fig. 3: CAPN7 downregulates the expression of HOXA10 and inhibits embryo implantation.

From: Calpain7 impairs embryo implantation by downregulating β3-integrin expression via degradation of HOXA10

Fig. 3: CAPN7 downregulates the expression of HOXA10 and inhibits embryo implantation.The alternative text for this image may have been generated using AI.

a Ishikawa cells were administered estrogen (10–8 M) and progesterone (10–6 M) at different times, as indicated. Whole-cell lysates were analyzed by western blot analysis with the indicated antibodies. The values of the density were marked above the band. b The Ishikawa cells transduced with Ad-GFP-CAPN7 (100 MOI)were treated with estrogen (E) (10–8 M) and progesterone (P) (10–6 M) at different times, as indicated. Whole-cell lysates were analyzed by western blot analysis with the indicated antibodies. The values of the density were marked above the band. c Ishikawa cells were transfected with ITGB3-Luc, Ad -Myc-HOXA10 (20 MOI), and Ad-GFP-CAPN7 (100 MOI), as indicated. After 48 h, the luciferase activities were measured and are presented as fold induction. Values represent the mean ± SEM (n = 3), *p < 0.05, **p < 0.01. d BeWo spheroids (150–200 μm diameter) were attached to Ishikawa cells after 2 h of co-culture. Adhesion experiments with BeWo spheroids attached to the Ishikawa cell monolayer. The data are the average of three independent experiments. An ANOVA test was used to compare the percentage of the attached spheroids with each treatment in comparison to the control. The error bars indicate SD of three independent experiments. Values represent the mean ± SEM (n = 3), *p < 0.05, **p < 0.01. e Ishikawa cells were transduced with Ad-GFP or Ad-GFP-CAPN7 (100 MOI) as indicated. Western blot analysis was performed with the indicated antibodies

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