Fig. 5: PEST motif 127-163 is critical for CAPN7-mediated HOXA10 degradation | Cell Death & Disease

Fig. 5: PEST motif 127-163 is critical for CAPN7-mediated HOXA10 degradation

From: Calpain7 impairs embryo implantation by downregulating β3-integrin expression via degradation of HOXA10

Fig. 5: PEST motif 127-163 is critical for CAPN7-mediated HOXA10 degradationThe alternative text for this image may have been generated using AI.

a, b Schematic model of generating the HOXA10 WT and HOXA10 PEST deletion mutant (HOXA10–dPEST) constructs. c Ishikawa cells transfected with Ad-GFP-CAPN7 and Ad-Flag-HOXA10 WT. Whole-cell lysates were detected by western blot analysis with the indicated antibodies. d Ishikawa cells transfected with Ad-GFP-CAPN7 and Ad-Flag-HOXA10 d127-163. Whole-cell lysates were detected by western blot analysis with the indicated antibodies. e Ishikawa cells transfected with Ad-GFP-CAPN7 and Ad-Flag-HOXA10 d292-311. Whole-cell lysates were detected by western blot analysis with the indicated antibodies. f CAPN7 degraded HOXA10 in vitro. g In vitro degradation assay, indicating that CAPN7 had a slight effect on HOXA10 d127-163. h Flag-CAPN7 degraded HOXA10 d292-311 in vitro. i The intensities of degraded HOXA10 signals were quantified from three independent experiments and normalized to the control group. Values represent the mean ± SEM (n = 3), ****p < 0.0001; **p < 0.01

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