Fig. 3 | Cell Death & Disease

Fig. 3

From: Cinacalcet-mediated activation of the CaMKKβ-LKB1-AMPK pathway attenuates diabetic nephropathy in db/db mice by modulation of apoptosis and autophagy

Fig. 3

Immunoblot for CaMKKβ, LKB1, phospho-AMPK, SIRT1 and phospho-Ser1177 eNOS in AMPKα1 siRNA, AMPKα2 siRNA, or SIRT1 siRNA knock-down HGECs in a high-glucose environment with cinacalcet treatment (a and b). The cultured HGECs were transfected with a final concentration of 50 nM CaMKKβ and LKB1, α1 and α2-AMPK, SIRT1 siRNAs for 24-h by transfection reagent and treated with cinacalcet (15 nM) in high-glucose media. Representative Western blot analyses of CaMKKβ and phospho-Ser428 LKB1 (a), as well as phospho-Thr172 AMPK, total AMPK, SIRT1, phospho-Ser1177 eNOS (b) and β-actin levels and the quantitative analyses of the results are also shown (a and b, respectively) (n = 4 independent experiments in each experiments).*P < 0.05, **P < 0.01 compared with control siRNA with HG

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