Figure 3: Non-classical monocytes exhibit several features of senescence. | Cell Death & Disease

Figure 3: Non-classical monocytes exhibit several features of senescence.

From: The pro-inflammatory phenotype of the human non-classical monocyte subset is attributed to senescence

Figure 3: Non-classical monocytes exhibit several features of senescence.

a Relative total cellular ROS levels as measured using H2DCFDA ROS indicator. b Relative mitochondrial ROS levels as measured using MitoSOX mitochondrial superoxide indicator. c–d Relative mitochondrial membrane potential (MMP) as measured using DIOC6 and JC-1. e Relative expression levels of p-ERK. All the parameters were measured using flow cytometry. Each line represents one donor; n ≥ 3. *p < 0.05; **p < 0.01; ****p < 0.0001. CL: classical, ITM: intermediate, NC: non-classical, MFI: median fluorescence intensity, MMP: mitochondrial membrane potential, ROS: reactive oxygen species

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