Fig. 2: Identification of il7r−/− homozygotes in F2 zebrafish. | Cell Death & Disease

Fig. 2: Identification of il7r−/− homozygotes in F2 zebrafish.

From: Knockout of zebrafish interleukin 7 receptor (IL7R) by the CRISPR/Cas9 system delays retinal neurodevelopment

Fig. 2: Identification of il7r−/− homozygotes in F2 zebrafish.

a PCR amplification of wild-type (#1), heterozygous (#2) and homozygous (#3) zebrafish. b-d Sequencing maps of wild-type (b), heterozygous (c) and homozygous (d) zebrafish. Red frames: sequences of the target site. Black frames: ACTG base pairs in wild-type and heterozygous zebrafish. Note the 4-bp (ACTG) deletion in homozygotes

Back to article page