Fig. 1: Elevated autophagy occurs in HCC cells treated with sorafenib. | Cell Death & Disease

Fig. 1: Elevated autophagy occurs in HCC cells treated with sorafenib.

From: PU.1/microRNA-142-3p targets ATG5/ATG16L1 to inactivate autophagy and sensitize hepatocellular carcinoma cells to sorafenib

Fig. 1: Elevated autophagy occurs in HCC cells treated with sorafenib.

a Western blotting was performed to measure autophagic activity in HCC cells treated with sorafenib and with or without Baf-A1. b Western blotting was performed to measure the autophagy levels in HCC cells treated with increasing concentrations of sorafenib and with or without Baf-A1. c SMMC-7721 and HepG2 cells were transfected with a GFP-LC3 plasmid, after which they were treated with the indicated concentrations of sorafenib for 24 h. At the end of treatment, the cells were inspected under a fluorescence microscope. d Transmission electron microscopy was utilized to determine the autophagosome levels. All data are presented as the mean ± S.D. from three independent experiments. The p-values represent comparisons between groups (**p < 0.01)

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