Fig. 5: Pharmacological blockade of P2Y1Rs increases NMDAR-induced current density in rat hippocampal neurons.
From: Glutamate-induced and NMDA receptor-mediated neurodegeneration entails P2Y1 receptor activation
a [3H]Aspartic acid saturation-specific binding curve to NMDARs in rat hippocampal synaptosomal membranes with a Bmax of 923.9 ± 110.5 fmol/mg protein and with a KD of 199 ± 48 nM (mean ± SEM; n = 7). The non-NMDAR binding was defined in the presence of D-AP-5 (30 µM). Neither MRS2179 (10 µM) nor MRS2500 (10 µM) affected the Bmax and the KD values of [3H]aspartic acid specific binding (bar graphs; n = 6). b Representative traces of NMDA-induced inward currents in rat hippocampal neurons in the absence and in the presence of MRS2179 (10 µM). c Rat hippocampal neurons exposed to MRS2179 (10 µM) for 30 min presented a higher NMDA-induced current density in relation to untreated cells. Data are expressed as the mean ± SEM of peak NMDA-induced current density (pA/pF). *p < 0.05 unpaired t test (n = 6–7)
