Fig. 5: DAX1 activated the Wnt/β-catenin pathway through directly transcriptionally repressing the expression of GSK3β in cervical cancer cells | Cell Death & Disease

Fig. 5: DAX1 activated the Wnt/β-catenin pathway through directly transcriptionally repressing the expression of GSK3β in cervical cancer cells

From: DAX1 promotes cervical cancer cell growth and tumorigenicity through activation of Wnt/β-catenin pathway via GSK3β

Fig. 5

. a The transcript levels of GSK3β, CCND1, C-MYC, and CDKNI1A in DAX1-silenced HeLa and SiHa cells were determined by real-time PCR. b The GSK3β promoter structure was constructed, and luciferase activity relative to Renilla control was measured in DAX1-silencing HeLa and SiHa cells. c, d The qChIP assay is shown in the DAX1-knockout HeLa and SiHa cells immunoprecipitation by GSK3β antibody and IgG antibody (as the negative control). e, f The expression of DAX1 and GSK3β was detected by IHC and real-time PCR; also, the correlation was analyzed. g The analysis of correlation between DAX1 and CTNNB1, GSK3β, CCND1, C-MYC, and other target genes of Wnt pathway. Data were obtained from GEO database and correlation analysis used Spearman rank correlation analysis. The two independent samples show mean ± SD and ttest was used to compare the means of samples. *P < 0.05

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