Fig. 7: Ceramide triggers BOK association with p-DRP1 and MFN2 and VDAC1 to IP3R at the mitochondria-associated ER membranes.
From: Ceramide-induced BOK promotes mitochondrial fission in preeclampsia
a Representative western blot and densitometry of MFN2 in HEK-293 cells stably transfected with GFP-BOK and induced with Dox (Dox: 0 (dH2O vehicle), 1.25 and 2.5 ng/mL, n = 4 separate experiments in duplicate; one-way ANOVA, Tukey’s post-test *P < 0.01). b Primary isolated cytotrophoblast cells treated with CER 16:0 or EtOH vehicle were stained for: (i/ii) BOK (green), calreticulin (red), and nuclear DAPI (blue) (n = 3 separate experiments); (iii/iv) p-DRP1 (green), calreticulin (red), and nuclear DAPI (blue) (n = 3 separate experiments); (v/vi) BOK (green), MFN2 (red), and nuclear DAPI (blue) (n = 3 separate experiments); and (vii/viii) p-DRP1 (green), MFN2 (red), and nuclear DAPI (blue) (n = 3 separate experiments). c Representative confocal images of in situ proximity ligation assay targeting VDAC1 and IP3R interactions in JEG3 cells exposed to CER 16:0 (20 μM) and 2-oleoylethanolamine (2-OE; 25 μM) for 6 h. Reactions without positive and negative PLA probes were used as negative controls
