Fig. 7: Dioscin suppresses Wnt/β-catenin pathway mainly by blocking the phosphorylation of Akt and GSK3. | Cell Death & Disease

Fig. 7: Dioscin suppresses Wnt/β-catenin pathway mainly by blocking the phosphorylation of Akt and GSK3.

From: Dioscin inhibits stem-cell-like properties and tumor growth of osteosarcoma through Akt/GSK3/β-catenin signaling pathway

Fig. 7

a The level of phosphorylated-Akt (Ser473), total Akt, phosphorylated-GSK3α/β (Ser21/9), total GSK3α/β and β-catenin were examined by western blot in U2OS cells treated with different concentrations of dioscin for 48 h. Dioscin blocked the phosphorylation of Akt and GSK3 in a dose-dependent manner. b The level of p-Akt (Ser473), p-GSK3β (Ser9) and β-catenin was detected by IHC staining in same tumor samples of mice treated with vehicle or dioscin 60 mg/kg. Scale bar, 100 μm. c The intensity of IHC staining (p-Akt (Ser473), p-GSK3β (Ser9) and β-catenin) was scored. Dioscin 60 mg/kg significantly decreased the level of p-Akt (Ser473), p-GSK3β (Ser9) and β-catenin in vivo. Data represent the mean ± SD of IHC staining scores of eight mice. d, e The level of p-Akt (Ser473), p-GSK3β (Ser9), and β-catenin was analyzed by immunofluorescence in U2OS and 143B cells treated with vehicle or 2.5 μm dioscin for 48 h. Scale bar, 10 μm. ***p < 0.001 by two-tailed Student’s ttest, SPSS 20.0

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