Fig. 4: Autophagy is impaired in Atg7-KO mouse kidney proximal tubular cells. | Cell Death & Disease

Fig. 4: Autophagy is impaired in Atg7-KO mouse kidney proximal tubular cells.

From: Histone deacetylase inhibitors protect against cisplatin-induced acute kidney injury by activating autophagy in proximal tubular cells

Fig. 4

Wild-type (Atg7 WT) and Atg7-knockout (Atg7 KO) MPTC cells were transiently transfected with mRFP-GFP-LC3 and then untreated or treated with cisplatin (20 μM), TSA (0.1 μM), or cisplatin+TSA for 20 h. a Representative images showing GFP-LC3 and mRFP-LC3 puncta (×630). Scale bar: 15 μm. b, c Quantitative analysis of GFP-LC3 puncta and RFP-LC3 puncta. d Analysis of autophagic flux rate. Data in b, c, d are expressed as mean ± SD. *P < 0.05, significantly different from the control group. #P < 0.05, significantly different from the cisplatin-only group. ^P < 0.05, significantly different from the corresponding group in Atg7 WT cells. e, f Atg7 WT and Atg7 KO MPTC cells were treated as described above without transfection. Whole-cell lysates were collected after treatment for immunoblot analysis of LC3B and ATG7. β-Actin was used as a loading control. e Representative immunoblots. f Densitometric analysis of LC3B signals. Data are expressed as mean ± SD. *P < 0.05, significantly different from the control group. #P < 0.05, significantly different from the cisplatin-only group. ^P < 0.05, significantly different from the corresponding group in Atg7 WT cells

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