Fig. 7: Targeting knockdown of CD44 mimicked the effects of galunisertib on invasive properties of HCC cells.

a Invasive growth assay in 3-D culture. Representative images of spheroids are shown. b Quantification of invasive growth capacity in 3-D culture. The increase of the area occupied by the spheroids between day 0 and day 4 was calculated. The line in the dot-plot graph represents mean value. *P < 0.05; **P < 0.001; ***P < 0.0001