Fig. 3: The loss of Synj1 does not alter the homeostasis of late endosomes in HeLa and SH-SY5Y cells. | Cell Death & Disease

Fig. 3: The loss of Synj1 does not alter the homeostasis of late endosomes in HeLa and SH-SY5Y cells.

From: Alteration of endosomal trafficking is associated with early-onset parkinsonism caused by SYNJ1 mutations

Fig. 3

Ctli and Synj1i HeLa a, b and SH-SY5Y c, d cells were stained with Rab7 antibody detected with Alexa-546-conjugated secondary antibodies. Serial confocal sections were collected from the top to the bottom of the cells. Representative images showing comparable late endosomes in Ctli and Synj1i cells a-d. Scale bars, 10 μm. Pictures at higher magnification (a1-d1) are shown; scale bars, 5 μm. e, f Mean fluorescence intensity (arbitrary unit, a.u.) in Ctli and Synj1i cells is shown. The bars show relative mean values ± SD of three independent experiments performed in pools and clones as described in Figs. 2e and n; n ≥ 50 cells. g-j Immunoblot detection of Rab7 g, i and densitometric analyses h, j of three different experiments performed in aforementioned stably interfered HeLa and SH-SY5Y cells. Calnexin and tubulin were used as loading control. The molecular weight of protein markers is indicated. Error bars, means ± SD

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