Fig. 5: The loss of Synj1 leads to intracellular accumulation of transferrin in SH-SY5Y cells. | Cell Death & Disease

Fig. 5: The loss of Synj1 leads to intracellular accumulation of transferrin in SH-SY5Y cells.

From: Alteration of endosomal trafficking is associated with early-onset parkinsonism caused by SYNJ1 mutations

Fig. 5

a-h Internalisation assay of Alexa-488-conjugated transferrin (Tf) in Ctli and Synj1i SH-SY5Y cells. Cells were pulsed with Tf for 7 min and then chased for different indicated times as described in Fig. 4. Representative single confocal sections show that Tf is uptaken similarly in control and silenced cells, whereas it is more intracellularly stalled in Synj1-interfered cells. Bars, 10 μm. Higher magnification pictures are shown in the insets. 3D reconstructions are shown in Supplementary Figure S6g-l. g Mean fluorescence intensity (arbitrary unit, a.u.) of Tf is shown. Experiments were performed three independent times in the two aforementioned pools. Error bars, means ± SD; n ≥ 50 cells, **p < 0.01, Student’s t-test. h Curves of Tf internalisation expressed as mean values of fluorescence measured at chase times compared with the fluorescent signal after pulse (set to 1) are shown (p < 0.001, Bonferroni test after significant ANOVA)

Back to article page