Fig. 8: Ectopic expression of wild-type Synj1 restores the proper trafficking of transferrin. | Cell Death & Disease

Fig. 8: Ectopic expression of wild-type Synj1 restores the proper trafficking of transferrin.

From: Alteration of endosomal trafficking is associated with early-onset parkinsonism caused by SYNJ1 mutations

Fig. 8

a-d Synj1i HeLa were transiently transfected with cDNA coding for wild-type Synj1 ( + Synj1, c, d) or untransfected (just electroporated, a, b). Forty-eight hours after transfection, cells were subjected to a Tf internalisation assay as described in Fig. 4. After fixation, cells were stained with a specific antibody anti-Synj1 (green). Representative images corresponding to 20 min chase of Tf a-d and mean fluorescence intensity (arbitrary unit, a.u.; e) in untransfected and transfected cells are shown. Error bars, means ± SD, n ≥ 30 cells; **p < 0.01, Student’s t-test. Scale bars, 5 μm

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